Journal:

Article Title: Analysis of dofA , a fruA -Dependent Developmental Gene, and Its Homologue, dofB , in Myxococcus xanthus

doi: 10.1128/JB.184.24.6803-6810.2002

Figure Lengend Snippet: Bacterial strains and plasmids used in this study

Article Snippet: pGEM-T easy , Ap r lacZ cloning vector , Promega.

Techniques: Plasmid Preparation, Clone Assay

Journal: American Journal of Physiology - Heart and Circulatory Physiology

Article Title: Angiopoietin-1 prevents severe bleeding complications induced by heparin-like drugs and fibroblast growth factor-2 in mice

doi: 10.1152/ajpheart.00373.2015

Figure Lengend Snippet: PPS did not affect the expression of metalloproteinases or VEGF-A in the intestine of mice infected with rAd-LacZ. A: representative pictures of the Western blots done with samples derived from the small intestinal of mice infected with rAd-LacZ vectors and treated with PPS (60 mg/kg) or phosphate-buffered saline (PBS). Only the intestinal expression of ICAM-1 was significantly increased in mice treated with rAd-LacZ + PPS relative to the control mice infected with rAd-LacZ (*P < 0.05; n = 6 samples per group). B: the densitometric analysis corresponding to the Western blots. Results are expressed in arbitrary optical density units as ratio of the beta-actin expression (means ± SE). Samples from 6 different mice were included in each group.

Article Snippet: Recombinant adenoviral (rAd) vectors. rAd-vectors carrying either the Escherichia coli LacZ gene (rAd-Lac-Z), a 700-bp cDNA sequence encoding a secreted form of human recombinant FGF-2 (rAd-FGF-2), or a genetically modified form of angiopoietin-1 (Ang-1*) from Regeneron Pharm (Tarrytown, NY), were generated, amplified, and purified as previously described ( 13 , 21 , 44 , 50 ).

Techniques: Expressing, Infection, Western Blot, Derivative Assay, Saline, Control

Journal: Molecular Plant Pathology

Article Title: The wxacO gene of Xanthomonas citri ssp. citri encodes a protein with a role in lipopolysaccharide biosynthesis, biofilm formation, stress tolerance and virulence

doi: 10.1111/j.1364-3703.2010.00681.x

Figure Lengend Snippet: Bacterial strains and plasmids. *

Article Snippet: However, in the glass tube CV staining assays, approximately 4.4‐fold and 2.8‐fold reductions in biofilm formation (absorbance at 590 nm) were observed in 247C8 ( wxacO ) and 236E9 ( rfbC ) compared with the wild‐type strain 306 ( ). table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Strains and plasmids Characteristics Reference or source Strains Escherichia coli DH5α F ‐ recA1 endA1 hsdR17 supE44 thi‐1 gyrA96 relA1 Δ ( argE‐lacZYA ) 169 ϕ80 lazA Δ M15 Hanahan (1983 ) JM109 rec A1, end A1, gyr A96, thi , hsd R17 (rK ‐ ,mK + ), rel A1, sup E44, Δ( lac ‐ pro AB), [F′, tra D36, pro AB, lac IqZΔM15] Promega HB101 F ‐ supE44, hsdS20(rB ‐ mB ‐ ), recA13, ara‐14, proA2, lacY1, galK2, rpsL20, xyl‐5, mtl‐1, leuB6, thi Boyer and Roulland‐Dussoix (1969 ) Xanthomonas citri ssp. citri 306 Rf r , cause citrus canker on citrus Rybak et al . (2009 ) 247C8 ( wxacO ) wxacO ( XAC3596 ): Tn5 derivative of strain 306, Rf r , Km r This study 236E9 ( rfbC ) rfbC ( XAC3598 ): Tn5 derivative of strain 306, Rf r , Km r This study 247C8V 247C8 ( wxacO ) containing pUFR053, Km r , Cm r , Gm r This study 236E9V 236E9 ( rfbC ) containing pUFR053, Km r , Cm r , Gm r This study C247C8 247C8 ( wxacO ) containing pJU3596, Km r , Cm r , Gm r This study C236E9 236E9 ( rfbC ) containing pJU3598, Km r , Cm r , Gm r This study Plasmids pGEM®‐T Easy PCR cloning and sequencing vector, lacZ ', Ap r Promega pRK2013 ColE1 Km r Tra + , conjugation helper plasmid Figurski and Helinski (1979 ) pUFR053 IncW Mob + mob (P) lac Zα + Par + , Cm r , Gm r , Km r , shuttle vector El Yacoubi et al . (2007 ) pUFZ75 Constitutive GFP expression vector; trp promoter cloned upstream of the GFP cassette; Km r Zhang et al . (2009 ) pGE3596 wxacO ( XAC3596 ) gene from Xcc 306 cloned in pGEM®‐T‐Easy This study pGE3598 rfbC ( XAC3598 ) gene from Xcc 306 cloned in pGEM®‐T‐Easy This study pJU3596 wxacO ( XAC3596 ) gene on Hin dIII fragment from pGE3596 cloned in pUFR053 This study pJU3598 rfbC ( XAC3598 ) gene on Hin dIII fragment from pGE3598 cloned in pUFR053 This study Open in a separate window * Ap r , Cm r , Km r , Gm r , Rf r and Tc r indicate resistance to ampicillin, chloromycetin, kanamycin, gentamicin, rifamycin and tetracycline, respectively.

Techniques: PCR Cloning, Sequencing, Plasmid Preparation, Conjugation Assay, Expressing, Clone Assay